The main focus of the Maguire lab is the development of more potent and safer gene delivery systemS to answer therapeutic challenges of today and tomorrow. One such technology is called exo-AAV. exo-AAV are enveloped adeno-associated virus (AAV) vectors. The envelope is derived from exosomes which are lipid based nanoparticles released by cells. Exosomes have several properties which may enhance AAV-mediated gene delivery.
From WIREs Nanomed Nanobiotechnol 2017, e1488. doi 10.1002/wnan.1488
Example of a exo-AAV. Note double layer membrane of exosome and at least 5 AAV capsids inside. Cryo-EM performed by Professor Alain Brisson and Sisareuth Tan.
Transmission electron microscopic image of purified AAV9 vector from our lab.
Purkinje neurons expressing GFP after intravenous gene delivery by exo-AAV. Blood vessels shown in red. Serial 2-photon tomography image by TissueVision, Inc.
Efficient gene delivery (GFP-green signal) after intravenous injection of exo-AAV. Image by Eloise Hudry.
Efficient gene expression (GFP) mediated by an AAV vector in sensory hair cells of the mouse inner ear. Hair cells are identified by myosin 7a staining (red). Image by Bence Gyorgy. Collaboration with David P. Corey, Harvard Medical School